Drastic change of local stiffness distribution correlating to cell migration in living fibroblasts.
نویسندگان
چکیده
Sequential images of the local stiffness distribution of living fibroblasts (NIH3T3) were captured under a culture condition using scanning probe microscopy in a force modulation mode. We found a clear relation between cell migration and local stiffness distribution on the cell: When cells were stationary at one position, the stiffness distribution of their cellular surface was quite stable. On the other hand, once the cells started to move, the stiffness in their nuclear regions drastically decreased. Possible explanations for the correlation between the cell migration and the cell stiffness are proposed.
منابع مشابه
The Role of Human Adult Peripheral and Umbilical Cord Blood Platelet-Rich Plasma on Proliferation and Migration of Human Skin Fibroblasts
BACKGROUND Wound healing is a complex and dynamic process following damage in tissue structures. Due to extensive skin damage caused by burn injuries, this study determined the role of human adult peripheral and umbilical cord blood platelet-rich plasma on proliferation and migration in human skin fibroblasts. METHODS Platelet-rich plasma (5, 10, 15, 20 and 50% PRP) from human umbilica...
متن کاملThe Effect of Short-Range Radiation of Type C and B Ultraviolet on the Mechanical Properties of Skin Fibroblasts
The effect of UV beam, which has been emitted from a natural or a manmade source on cells has been studied in previous studies for several times. Radiation of this beam can have different effects on DNA of the cell, cytotoxicity, the structure of cellular proteins and their mechanical properties based on radiation period or frequency. The effect of radiation of two types of beams, namely UVB an...
متن کاملApplication and Assessment of Allogeneic Fibroblasts for Cell Therapy
Background and Objective: In recent years, due to increasing number of patients with non-healing skin ulcers, skin substitutes have been used. Skin substitutes contain living cells causing faster and more effective wound healing. Therefore, research on the use of autologous and allogeneic cells such as fibroblasts in skin substitutes has attracted attentions. However, there are...
متن کاملMiro1-mediated mitochondrial positioning shapes intracellular energy gradients required for cell migration
It has long been postulated, although never directly demonstrated, that mitochondria are strategically positioned in the cytoplasm to meet local requirements for energy production. Here we show that positioning of mitochondria in mouse embryonic fibroblasts (MEFs) determines the shape of intracellular energy gradients in living cells. Specifically, the ratio of ATP to ADP was highest at perinuc...
متن کاملEffect of Stress-Fiber Inclusion on the Local Stiffness of Cell Cytoskeleton Probed by AFM Indentation: Insights from a Discrete Network Model
In this paper, we analyze the effect of stress-fiber inclusion on the stiffness of an actin random network. To do this, use a discrete random network model to analyze the elastic response of this system in terms of apparent Young’s modulus. First, we showed that for a flat-ended cylindrical AFM indenter the total indentation force has a linear relation with the indentation depth and the indente...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Cell motility and the cytoskeleton
دوره 50 4 شماره
صفحات -
تاریخ انتشار 2001